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intercepttm tbs blocking buffer  (LI-COR)


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    Structured Review

    LI-COR intercepttm tbs blocking buffer
    Intercepttm Tbs Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 2135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercepttm+tbs+blocking+buffer/Intercept+(TBS)+Blocking+Buffer/bio_rxiv__64898__2026__03__23__713706-191-26-30
    Average 98 stars, based on 2135 article reviews
    intercepttm tbs blocking buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Early divergent modulation of NLRP2's and NLRP3's inflammasome sensors vs. AIM2's one by signals from Aβ·Calcium-sensing receptor complexes in human astrocytes.
    Article Snippet: Next, the lysates were loaded on a NuPAGETM 4–12 % (Cat. No. NP0321BOX) or 10 % Bis-Tris polyacrylamide gel (Cat. No. NP0301BOX) (all from Thermo Fisher Scientific). .. After electrophoresis in NuPAGETM MES SDS Running Buffer (Cat. No. NP0002) using the XCell SureLockTM Mini-Cell, proteins were blotted onto nitrocellulose membranes using the iBlotTM Dry Blotting System (all from Thermo Fisher Scientific) and blocked with InterceptTM TBS blocking buffer (Cat. No. 927–60001, LI-COR) for 1 h at room temperature. ..

    Blocking Assay:

    Article Title: Early divergent modulation of NLRP2's and NLRP3's inflammasome sensors vs. AIM2's one by signals from Aβ·Calcium-sensing receptor complexes in human astrocytes.
    Article Snippet: Next, the lysates were loaded on a NuPAGETM 4–12 % (Cat. No. NP0321BOX) or 10 % Bis-Tris polyacrylamide gel (Cat. No. NP0301BOX) (all from Thermo Fisher Scientific). .. After electrophoresis in NuPAGETM MES SDS Running Buffer (Cat. No. NP0002) using the XCell SureLockTM Mini-Cell, proteins were blotted onto nitrocellulose membranes using the iBlotTM Dry Blotting System (all from Thermo Fisher Scientific) and blocked with InterceptTM TBS blocking buffer (Cat. No. 927–60001, LI-COR) for 1 h at room temperature. ..

    Article Title: The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover.
    Article Snippet: .. Secondary antibodies were diluted in InterceptTM (TBS) Blocking Buffer (927-60003, LI-COR) and incubated on the membrane for 1 h at room temperature. ..

    Article Title: Oncogenic E3-ligase adaptors MAGE-A3/6 promote cancer cell migration via BAP18 degradation
    Article Snippet: .. Nitrocellulose membranes were blocked in 5% (w/v) NFDM (MilliporeTM, 70166) in TBS-T with 0,05% Tween 20 (Bio-Rad, #1706435 and #1610781) and LF-PVDF membranes were blocked in InterceptTM (TBS) blocking buffer (LI-COR, #927-60001) for 3 hours at room temperature. .. Membranes were cut into appropriate molecular weight range slices and incubated with primary antibodies over night at 4°C, using 5 % (w/v) NFDM in TBS-T for chemiluminescence detection and Intercept® (TBS) antibody dilution buffer (LI-COR, #927-65001).

    Article Title: Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.
    Article Snippet: .. Cells were blocked at RT for 1 h in InterceptTM (TBS) Blocking Buffer (927-60003, LI-COR) plus 0.3% Triton X-100, then washed once in 1× DPBS. ..

    Article Title: The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover.
    Article Snippet: Cells were washed twice in warm 1X HBSS and fixed for 15 min in 4% paraformaldehyde (PFA) made from fresh 16% PFA (#15710, Electron Microscopy Sciences) diluted with 1× HBSS. .. Cells were blocked and permeabilized at room temperature for 1 h in InterceptTM (TBS) Blocking Buffer (927-60003, LI-COR) plus 0.3% Triton X-100, then washed once in 1× HBSS. ..

    Article Title: Early divergent modulation of NLRP2's and NLRP3's inflammasome sensors vs. AIM2's one by signals from Aβ·Calcium-sensing receptor complexes in human astrocytes.
    Article Snippet: The protein contents of the samples were quantified by using the Bio-Rad Protein Assay (Cat. No. 500–0006, Bio-Rad). .. Briefly, equal amounts (100 μg) of protein extracts were then incubated with the antibody array membranes previously treated with InterceptTM TBS blocking buffer (Cat. No. 927–60001, LI-COR GmbH, Bad Homburg, Germany) for 1-h. After overnight incubation at 4 ◦C, the array membranes were washed and incubated for 2 h with the RayBioTM Detection Antibody Cocktail. ..

    Article Title: Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.
    Article Snippet: For Western blotting, samples were transferred for 60 min to 0.2 μm PVDF membranes (#ISEQ00010, Sigma) using a Semi-dry transfer cell (Bio-Rad). .. Membranes were blocked for 20 min in InterceptTM (TBS) Blocking Buffer (927-60003, LI-COR). ..

    Article Title: DCPS is a synthetic lethal therapeutic target in acute myeloid leukemia expressing low levels of FHIT
    Article Snippet: Nitrocellulose membranes (Thermo Fisher Scientific) were used for transfer using the iBlotTM 2 Dry Blotting System (Thermo Fischer Scientific). .. Membranes were blocked in InterceptTM (TBS) Blocking Buffer (LI-COR Biosciences) for 60 minutes room temperature (RT) and incubation with primary antibody was performed overnight (ON) at 4°C. .. After 3x washing steps with TBS-T supplemented with 0.05% Tween-20 (Sigma-Aldrich, Bulington, MA, USA), membranes were incubated with secondary antibodies for 60 minutes.

    Incubation:

    Article Title: The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover.
    Article Snippet: .. Secondary antibodies were diluted in InterceptTM (TBS) Blocking Buffer (927-60003, LI-COR) and incubated on the membrane for 1 h at room temperature. ..

    Article Title: Early divergent modulation of NLRP2's and NLRP3's inflammasome sensors vs. AIM2's one by signals from Aβ·Calcium-sensing receptor complexes in human astrocytes.
    Article Snippet: The protein contents of the samples were quantified by using the Bio-Rad Protein Assay (Cat. No. 500–0006, Bio-Rad). .. Briefly, equal amounts (100 μg) of protein extracts were then incubated with the antibody array membranes previously treated with InterceptTM TBS blocking buffer (Cat. No. 927–60001, LI-COR GmbH, Bad Homburg, Germany) for 1-h. After overnight incubation at 4 ◦C, the array membranes were washed and incubated for 2 h with the RayBioTM Detection Antibody Cocktail. ..

    Article Title: DCPS is a synthetic lethal therapeutic target in acute myeloid leukemia expressing low levels of FHIT
    Article Snippet: Nitrocellulose membranes (Thermo Fisher Scientific) were used for transfer using the iBlotTM 2 Dry Blotting System (Thermo Fischer Scientific). .. Membranes were blocked in InterceptTM (TBS) Blocking Buffer (LI-COR Biosciences) for 60 minutes room temperature (RT) and incubation with primary antibody was performed overnight (ON) at 4°C. .. After 3x washing steps with TBS-T supplemented with 0.05% Tween-20 (Sigma-Aldrich, Bulington, MA, USA), membranes were incubated with secondary antibodies for 60 minutes.

    Membrane:

    Article Title: The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover.
    Article Snippet: .. Secondary antibodies were diluted in InterceptTM (TBS) Blocking Buffer (927-60003, LI-COR) and incubated on the membrane for 1 h at room temperature. ..

    Ab Array:

    Article Title: Early divergent modulation of NLRP2's and NLRP3's inflammasome sensors vs. AIM2's one by signals from Aβ·Calcium-sensing receptor complexes in human astrocytes.
    Article Snippet: The protein contents of the samples were quantified by using the Bio-Rad Protein Assay (Cat. No. 500–0006, Bio-Rad). .. Briefly, equal amounts (100 μg) of protein extracts were then incubated with the antibody array membranes previously treated with InterceptTM TBS blocking buffer (Cat. No. 927–60001, LI-COR GmbH, Bad Homburg, Germany) for 1-h. After overnight incubation at 4 ◦C, the array membranes were washed and incubated for 2 h with the RayBioTM Detection Antibody Cocktail. ..



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